Description
Intended Uses: This sandwich kit is for the accurate quantitative detection of Mouse A Disintegrin and Metalloproteinase with Thrombospondin Motifs 8 (also known as ADAMTS8) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse ADAMTS8 antibody. ADAMTS8 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse ADAMTS8 Antibody is added and binds to ADAMTS8 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ADAMTS8 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse ADAMTS8. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm